Reconstituting a peptide means dissolving a freeze-dried (lyophilized) peptide in a solvent to create a usable solution. This must be done carefully to preserve the peptide’s stability.
Gather Supplies:
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Lyophilized (freeze dried) peptide.
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Bacteriostatic water (or other).
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Sterile syringe/needle.
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Alcohol wipes for sterilization.
Calculate Solvent Volume:
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Use our Peptide Calculator
Sterilize:
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Clean the vial and syringe with alcohol wipes.
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Work in a clean environment to avoid contamination.
Add Bacteriostatic water:
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Draw the calculated bacteriostatic water volume into the syringe.
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Slowly inject it along the vial’s inner wall to avoid disturbing the peptide (the vial will be at a negative pressure, so it will want to draw the bacteriostatic water in, be prepared to slow down the injection into the vial with pressure against the plunger).
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Gently roll the vial (don’t shake) to dissolve. Let it sit if needed.
Store Properly:
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Store at 2°C and 4°C and away from sunlight.
Key Tips:
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Peptide Care: Avoid shaking or excessive heat. Some peptides need specific conditions (e.g., pH or reducing agents).
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Dosage: After reconstitution, calculate doses based on concentration. Example: For a 2 mg/mL solution, 0.1 mL = 200 µg.
- Safety: Research peptides are not for human use.
Always consult with a healthcare professional for personalized guidance and training before performing injections.